Etoposide (VP-16)

Catalog No. A10373

Etoposide (VP-16)是通过抑制topoisomerase II活性而抑制DNA合成。

Product Citations

Reiko Watanabe, .et al. Development of an In Silico Prediction Model for P-glycoprotein Efflux Potential in Brain Capillary Endothelial Cells toward the Prediction of Brain Penetration, J Med Chem, 2021, Mar 11;64(5):2725-2738 PMID: 33619967
Chen JC, .et al. In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells, Biomolecules, 2019, Sep 9;9(9) PMID: 31505885
Takashi Azuma, .et al. Removal of pharmaceuticals in water by introduction of ozonated microbubbles, SEP PURIF TECHNOL, 2019, Apr; 212: 483-489
Calhelha RC, .et al. Mathematical models of cytotoxic effects in endpoint tumor cell line assays: critical assessment of the application of a single parametric value as a standard criterion to quantify the dose-response effects and new unexplored proposal formats, Analyst, 2017, Oct 23;142(21):4124-4141 PMID: 28991301

Technical details

Catalog Num	A10373
M. Wt	588.6
Formula	C29H32O13
Purity	>98%
Storage	at -20°C 3 years Powder
CAS No.	33419-42-0
Synonyms	VP16, Vepesid, etoposide phosphate
SMILES	C[C@@H]1OC[C@@H]2[C@@H](O1)[C@@H]([C@H]([C@@H](O2)O[C@H]3[C@H]4COC(=O)[C@@H]4[C@@H](C5=CC6=C(C=C35)OCO6)C7=CC(=C(C(=C7)OC)O)OC)O)O

Biological Activity

Etoposide (VP-16)是通过抑制topoisomerase II活性而抑制DNA合成。

Targets

Topo II (Cell-free assay)

Solubility *

In vitro (25°C)	DMSO	99 mg/mL (168.2 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	5% DMSO+40% PEG 300+5% Tween 80+ddH2O	14 mg/mL
* <1 mg/ml means slightly soluble or insoluble. * Please note that Adooq tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Preparing Stock Solutions

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	16.99 mL	84.95 mL	169.89 mL
0.5 mM	3.4 mL	16.99 mL	33.98 mL
1 mM	1.7 mL	8.49 mL	16.99 mL
5 mM	0.34 mL	1.7 mL	3.4 mL

*The above data is based on the productmolecular weight 588.6. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.