Staurosporine

Catalog No. A10864

Staurosporine是一种天然产物，最初于1977年从Streptomyces staurosporeus细菌中分离出来。星形孢菌素的主要生物学活性是通过防止ATP与激酶的结合来抑制蛋白激酶。是一种有效的PKC抑制剂，在无细胞试验中作用于PKCα，PKCγ 和PKCη，IC50分别为2 nM，5 nM 和 4 nM，对PKCδ(20 nM)和PKCε(73 nM作用较弱，对PKCζ (1086 nM)的活性很低。同时对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。

Product Citations

Lisa M Kim, .et al. Sustained Oncogenic Signaling in the Cytostatic State Enables Targeting of Nonproliferating Persistent Cancer Cells, Cancer Res, 2022, Sep 2;82(17):3045-3057 PMID: 35792658
Jacobs CL, .et al. StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins, Nat Methods, 2018, Jul;15(7):523-526 PMID: 29967496
Birte Plitzko, .et al. The natural product mensacarcin induces mitochondrial toxicity and apoptosis in melanoma cells, J Biol Chem, 2017, Dec 22; 292(51): 21102-21116 PMID: 29074620

Technical details

Catalog Num	A10864
M. Wt	466.5
Formula	C28H26N4O3
Purity	>98%
Storage	at -20°C 3 years Powder
CAS No.	62996-74-1
Synonyms	AM-2282, STS
SMILES	C[C@@]12[C@@H]([C@@H](C[C@@H](O1)N3C4=CC=CC=C4C5=C6C(=C7C8=CC=CC=C8N2C7=C53)CNC6=O)NC)OC

Biological Activity

Targets

Target	Value
PKCα	IC50: 2nM
c-Fgr	IC50: 2nM
phosphorylasekinase	IC50: 3nM
PKCη	IC50: 4nM
PKCγ	IC50: 5nM
S6kinase	IC50: 5nM
v-Src	IC50: 6nM
Cdc2	IC50: 9nM
Cdc2	IC50: 9nM
PKA	IC50: 15nM
Syk	IC50: 16nM
PKG	IC50: 18nM
PKCδ	IC50: 20nM
CaMKII	IC50: 20nM
Lyn	IC50: 20nM
MLCK	IC50: 21nM
PKCε	IC50: 73nM
PKCζ	IC50: 1086nM

Solubility *

In vitro	DMSO	4 mg/mL (8.57 mM)
	Water	Insoluble
	Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Adooq tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Preparing Stock Solutions

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	21.44 mL	107.18 mL	214.36 mL
0.5 mM	4.29 mL	21.44 mL	42.87 mL
1 mM	2.14 mL	10.72 mL	21.44 mL
5 mM	0.43 mL	2.14 mL	4.29 mL

*The above data is based on the productmolecular weight 466.5 . Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.