Nitro blue tetrazolium chloride
Catalog No. A21323
Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.
| Catalog Num | A21323 |
|---|---|
| M. Wt | 817.64 |
| Formula | C40H30Cl2N10O6 |
| Purity | >98% |
| Storage | at -20°C 3 years Powder |
| CAS No. | 298-83-9 |
| Synonyms | NBT |
| SMILES | COC1=CC(C2=CC=C(N3N=C(C4=CC=CC=C4)N=[N+]3C5=CC=C([N+]([O-])=O)C=C5)C(OC)=C2)=CC=C1N6N=C(C7=CC=CC=C7)N=[N+]6C8=CC=C([N+]([O-])=O)C=C8.[Cl-].[Cl-] |
Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 12.23 mL | 61.15 mL | 122.3 mL |
| 0.5 mM | 2.45 mL | 12.23 mL | 24.46 mL |
| 1 mM | 1.22 mL | 6.12 mL | 12.23 mL |
| 5 mM | 0.24 mL | 1.22 mL | 2.45 mL |
*The above data is based on the productmolecular weight 817.64. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
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