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20(S)-hydroxyCholesterol (20α-Hydroxycholesterol) is an allosteric activator of the oncoprotein smoothened (Smo) that activates the hedgehog (Hh) signaling pathway with an EC50 of 3 μM in a gene transcription reporter assay using NIH3T3 cells.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 24.84 mL | 124.18 mL | 248.35 mL |
| 0.5 mM | 4.97 mL | 24.84 mL | 49.67 mL |
| 1 mM | 2.48 mL | 12.42 mL | 24.84 mL |
| 5 mM | 0.5 mL | 2.48 mL | 4.97 mL |
*The above data is based on the productmolecular weight 402.65. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 目录号 | A21850 |
|---|---|
| 作用机制 | Activator (激活剂) |
| M. Wt | 402.65 |
| Formula | C27H46O2 |
| Purity | >99% |
| Storage | Store lyophilized at -20ºC, keep desiccated. |
| CAS No. | 516-72-3 |
| Synonyms | 20α-Hydroxycholesterol |
| SMILES | C[C@@]1([C@@]2([H])[C@@](C)(O)CCCC(C)C)[C@](CC2)([H])[C@@](CC=C3[C@@]4(CC[C@H](O)C3)C)([H])[C@]4([H])CC1 |
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