3X FLAG peptide

3X FLAG Peptide is a synthetic peptide with a 3-time repeated DYKXXD motif. 3X FLAG Peptide often used to competitively bind Anti-Flag antibodies.3X Flag Peptide is mainly used for protein identification and purification, such as elution of Flag fusion expression proteins bound to Anti-Flag antibodies during immunoprecipitation[1-5].

[1] Hopp, T., Prickett, K., et al. (1988). A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification. Nat Biotechnol, 6(10), 1204-1210. doi.org/10.1038/nbt1088-1204

[2] Einhauer, A., & Jungbauer, A. (2001). Affinity of the monoclonal antibody M1 directed against the FLAG peptide. J Chromatogr A, 921(1), 25-30. doi.org/10.1016/s0021-9673(01)00831-7 PMID: 11461009

[3] Gao, X. K., Rao, X. S., et al. (2022). Phase separation of insulin receptor substrate 1 drives the formation of insulin/IGF-1 signalosomes. Cell Discov, 8(1), 60. doi.org/10.1038/s41421-022-00426-x PMID: 35764611

[4] Gao, J., Huang, G., et al. (2022). PROTEIN S-ACYL TRANSFERASE 13/16 modulate disease resistance by S-acylation of the nucleotide binding, leucine-rich repeat protein R5L1 in Arabidopsis. J Integr Plant Biol, 64(9), 1789-1802. doi.org/10.1111/jipb.13324 PMID: 35778928

[5] Lian, H., Jiang, K., et al. (2021). The Salmonella effector protein SopD targets Rab8 to positively and negatively modulate the inflammatory response. Nat Microbiol, 6(5), 658-671. doi.org/10.1038/s41564-021-00866-3 PMID: 33603205