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Granulocyte-Colony Stimulating Factor Human Recombinant, CHO
| 目录号 | AP3356 |
|---|---|
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | G-CSF was lyophilized from a concentrated (1mg/ml) solution containing 10mM Hydrochloric Acid pH=6.5, 0.4mg tween 20, 100mg mannitol, 160mg L-arginine, 40mg phenylalanine and 4mg methionine. |
| Protein biological activity | The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.07 ng/ml, corresponding to a Specific Activity of 1.27 x 108 IU/mg. |
| Amino acid sequence | TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP. |
| Uniprot ACC# | P09919 |
| Purity | Greater than 97.0% as determined by SDS-PAGE. |
| Solubility | It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18M??-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Granulocyte Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution G-CSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Source | Chinese Hamster Ovary Cells (CHO). |
| Synonyms | CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF. |
| Transportation method | Shipped at Room temp |
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