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Transferrin Human Recombinant
| 目录号 | AP2547 |
|---|---|
| Applications | Recombinant Human Transferrin has increased the growth of numerous cell types including hybridoma and primary cell lines. The optimal concentration of Recombinant Human Transferrin for cell culture differs with the cell line. In CHO, effective concentrations range from 10-50 mg/liter. The reported effective concentrations for hybridoma and Vero cell lines range from 5mg/liter to 20mg/liter. Effective concentrations for primary cells range between 5mg/liter to 100mg/liter. |
| Physical Appearance | Sterile Filtered lyophilized (freeze-dried) powder. |
| Formulation | The protein (1mg/ml) was lyophilized with no additives. |
| Protein biological activity | One mg of Recombinant Human Transferrin will bind to approximately 2 micrograms of Fe. |
| Uniprot ACC# | P02787 |
| Purity | Purity as determined by SDS-PAGE is 95%. |
| Solubility | Stock solutions can be prepared by dissolving gently into PBS for several minutes. Recommended stock concentrations are 5mg/ml to 20 mg/ml in PBS, though others can be used as well. Please try to avoid the formation of bubbles when dissolving the protein. Sterile filter through 0.2?m filter. |
| Stability | Lyophilized Transferrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Transferrin Human Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Source | Oryza sativa (rice). |
| Synonyms | Serotransferrin, Transferrin, Siderophilin, Beta-1-metal-binding globulin, TF, PRO1557, PRO2086, DKFZp781D0156, HTF. |
| Transportation method | Shipped at Room temp |
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